Abstract
A phthalide glycoside, (3R, 4R)-4-O-β-D-glucopyranosyl-senkyunolide (1), and a megastigmane glycoside, (6S, 7R)-3-oxo-megastigma-4, 8-dien-7-O-β-D-glucoside (2), along with two known aglycones (3-4), were isolated from the 70% EtOH extract of fresh whole grass of Apium graveolens L. Their structures were elucidated by extensive spectroscopic analysis. All of these compounds were tested for their inhibitory effects on nitric oxide (NO) production in the RAW 264.7 macrophages. Among them, compounds 3 and 4 showed potent inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages, with IC50 values of 24.0 ± 2.1 μM and 28.6 ± 2.4 μM, respectively.
Keywords:
Apium graveolens; megastigmane glycoside; nitric oxide; phthalide glycoside.
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
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Apium / chemistry*
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Benzofurans / chemistry
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Benzofurans / isolation & purification*
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Benzofurans / pharmacology*
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Glucosides
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology*
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Inhibitory Concentration 50
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Mice
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Molecular Structure
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Nitric Oxide / biosynthesis
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Norisoprenoids
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Nuclear Magnetic Resonance, Biomolecular
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Plant Extracts / chemistry
Substances
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(3R,4R)-4-O-beta-D-glucopyranosyl-senkyunolide
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(6S,7R)-3-oxo-megastigma-4,8-dien-7-O-beta-D-glucoside
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Anti-Inflammatory Agents, Non-Steroidal
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Benzofurans
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Drugs, Chinese Herbal
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Glucosides
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Glycosides
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Lipopolysaccharides
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Norisoprenoids
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Plant Extracts
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Nitric Oxide