Acknowledgments
The authors would like to thank Valeria Bellini (Host-Pathogen Interactions and Immunity to Infections, IAB – Institute for Advanced Biosciences, Grenoble Alpes University, Grenoble, France) for the in vitro T. gondii culture.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by the Parasitology-Mycology Laboratory of Grenoble University Hospital Centre. The Parasitology-Mycology Laboratory of Grenoble University Hospital Centre received research grants from Roche®, Abbott® and bioMérieux®.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Robert-Gangneux F, Darde M-L. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 2012;25:264–96.10.1128/CMR.05013-11Search in Google Scholar PubMed PubMed Central
2. Dard C, Marty P, Brenier-Pinchart M-P, Garnaud C, Fricker-Hidalgo H, Pelloux H, et al. Management of toxoplasmosis in transplant recipients: an update. Expert Rev Anti Infect Ther 2018;16:447–60.10.1080/14787210.2018.1483721Search in Google Scholar PubMed
3. Brenier-Pinchart M-P, Capderou E, Bertini R-L, Bailly S, Fricker-Hidalgo H, Varlet-Marie E, et al. Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii. Diagn Microbiol Infect Dis 2015;82:289–91.10.1016/j.diagmicrobio.2015.04.004Search in Google Scholar PubMed
4. Robert-Gangneux F, Meroni V, Dupont D, Botterel F, Garcia JM, Brenier-Pinchart M-P, et al. Toxoplasmosis in transplant recipients, Europe, 2010–2014. Emerg Infect Dis 2018;24:1497–504.10.3201/eid2408.180045Search in Google Scholar PubMed PubMed Central
5. Yera H, Filisetti D, Bastien P, Ancelle T, Thulliez P, Delhaes L. Multicenter comparative evaluation of five commercial methods for Toxoplasma DNA extraction from amniotic fluid. J Clin Microbiol 2009;47:3881–6.10.1128/JCM.01164-09Search in Google Scholar PubMed PubMed Central
6. Faucher B, Miermont F, Ranque S, Franck J, Piarroux R. Optimization of Toxoplasma gondii DNA extraction from amniotic fluid using NucliSENS easyMAG and comparison with QIAamp DNA minikit. Eur J Clin Microbiol Infect Dis 2012;31:1035–9.10.1007/s10096-011-1402-zSearch in Google Scholar PubMed
7. Yera H, Ménégaut L, Brenier-Pinchart M-P, Touafek F, Bastien P, Dalle F, et al. Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid. Clin Microbiol Infect 2018;24:1100.e7–11.10.1016/j.cmi.2018.01.014Search in Google Scholar PubMed
8. Varlet-Marie E, Sterkers Y, Brenier-Pinchart M-P, Cassaing S, Dalle F, Delhaes L, et al. Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays. J Clin Microbiol 2014;52:3952–9.10.1128/JCM.01906-14Search in Google Scholar PubMed PubMed Central
9. Dien Bard J, Alby K. Point-Counterpoint: Meningitis/Encephalitis Syndromic Testing in the Clinical Laboratory. Ledeboer NA, éditeur. J Clin Microbiol 2018;56:1–10.10.1128/JCM.00018-18Search in Google Scholar
10. Roux G, Varlet-Marie E, Bastien P, Sterkers Y. Evolution of Toxoplasma-PCR methods and practices: a French national survey and proposal for technical guidelines. Int J Parasitol 2018;48:701–7.10.1016/j.ijpara.2018.03.011Search in Google Scholar PubMed
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