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Licensed Unlicensed Requires Authentication Published by De Gruyter December 10, 2019

Evaluation of PFA-100 closure times in cord blood samples of healthy term and preterm neonates

  • Serena Valsami , Maria Kollia , Vasiliki Mougiou , Rozeta Sokou , Elina Isaakidou , Maria Boutsikou , Abraham Pouliakis , Zoe Iliodromiti , Robert Carr , Theodora Boutsikou , Nicoletta Iacovidou and Marianna Politou EMAIL logo

Corresponding author: Assoc. Prof. Marianna Politou, MD, PhD, Hematology- Immunohematology, Head of Haematology Laboratory-Blood Bank, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Street, 11526 Athens, Greece, Phone: +0030 210 7286301, Fax: +0030 210 7230785
aSerena Valsami and Maria Kollia share first authorship.
  1. Author contributions: SV and MK: wrote the manuscript; NI and MP: designed the study; MK, VM and EI: performed the sample collection and analysis; RS, NI: supervised sample collection; SV and MP: supervised the sample analysis; MB and AP: performed the statistical analysis; RS, ZI, RC, TB, NI, MP: critically revised the manuscript. All authors have accepted responsibility for the entire content of the submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. Saxonhouse MA, Sola MC. Platelet function in term and preterm neonates. Clin Perinatol 2004;31:15–28.10.1016/j.clp.2004.03.009Search in Google Scholar PubMed

2. Carcao MD, Blanchette VS, Dean JA, He L, Kern MA, Stain AM, et al. The Platelet Function Analyzer (PFA-100): a novel in-vitro system for evaluation of primary haemostasis in children. Br J Haematol 1998;10:70–3.10.1046/j.1365-2141.1998.00656.xSearch in Google Scholar PubMed

3. Israels SJ, Cheang T, McMillan-Ward EM, Cheang M. Evaluation of primary hemostasis in neonates with a new in vitro platelet function analyzer. J Pediatr 2001;138:116–9.10.1067/mpd.2001.109794Search in Google Scholar PubMed

4. Boudewijns M, Raes M, Peeters V, Mewis A, Cartuyvels R, Magerman K, et al. Evaluation of platelet function on cord blood in 80 healthy term neonates using the Platelet Function Analyser (PFA-100); shorter in vitro bleeding times in neonates than adults. Eur J Pediatr 2003;162:212–3.10.1007/s00431-002-1093-7Search in Google Scholar PubMed

5. Roschitz B, Sudi K, Köstenberger M, Muntean W. Shorter PFA-100 closure times in neonates than in adults: role of red cells, white cells, platelets and von Willebrand factor. Acta Paediatr 2001;90:664–70.10.1007/978-3-642-59383-3_11Search in Google Scholar

6. Kundu SK, Heilmann EJ, Sio R, Garcia C, Davidson RM, Ostgaard RA. Description of an in vitro platelet function analyzer PFA-100. Semin Thromb Hemost 1995;21:106–12.10.1055/s-0032-1313612Search in Google Scholar PubMed

7. Saxonhouse MA, Garner R, Mammel L, Li Q, Muller KE, Greywoode J, et al. Closure times measured by the platelet function analyzer PFA-100 are longer in neonatal blood compared to cord blood samples. Neonatology 2010;97:242–9.10.1159/000253755Search in Google Scholar PubMed

8. Cho YU, Jang S, Park CJ, Chi HS. Variables that affect platelet function analyzer-100 (PFA-100) closure times and establishment of reference intervals in Korean adults. Ann Clin Lab Sci 2008;38:247–53.Search in Google Scholar

9. Rajasekhar D, Barnard MR, Bednarek FJ, Michelson AD. Platelet hyporeactivity in very low birth weight neonates. Thromb Haemost 1997;77:1002–7.10.1055/s-0038-1656093Search in Google Scholar

10. Deschmann E, Sola-Visner M, Saxonhouse MA. Primary hemostasis in neonates with thrombocytopenia. J Pediatr 2014;164:167–72.10.1016/j.jpeds.2013.08.037Search in Google Scholar PubMed

Received: 2019-09-08
Accepted: 2019-11-06
Published Online: 2019-12-10
Published in Print: 2020-03-26

©2020 Walter de Gruyter GmbH, Berlin/Boston

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