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Licensed Unlicensed Requires Authentication Published by De Gruyter May 12, 2021

Exercise-induced changes in miRNA expression in coronary artery disease

  • Barbara Mayr ORCID logo , Edith E. Müller , Christine Schäfer , Silke Droese , Martin Schönfelder and Josef Niebauer EMAIL logo

Abstract

Objectives

Micro ribonucleic acids (miRNAs) are small non-coding RNA molecules that control gene expression by translational inhibition. Exercise has been shown to affect several miRNAs’ expression in healthy subjects, but this has not yet been studied in patients with coronary artery disease (CAD). Since exercise training confers beneficial long-term effects and may also trigger acute coronary events, it is of utmost interest to be able to identify those who are risk for untoward effects. Therefore, we set out to assess miRNA expression in response to maximal ergospirometry in patients with CAD.

Methods

Total RNA was extracted from blood drawn immediately before and 5 min after maximal cycle-ergospirometry (10 male and 10 female CAD patients). A qRT-PCR was performed for 187 target miRNAs associated with endothelial function/dysfunction, cardiovascular disease, myocardial infarction, and sudden cardiac death.

Results

In response to a maximal ergospirometry, 33 miRNAs significantly changed their expression levels. Of these miRNAs 16 were significantly differently expressed between gender. Using multi-variance analysis, nine miRNAs (let-7e-5p; miR-1; miR-19b-1-5p; miR-103a-3p; miR-148b-3p; miR-181b-5p; miR-188-5p; miR-423-5p; miR-874-3p) showed significantly different responses to maximal ergospirometry between genders.

Conclusions

We report for the first time that in patients with CAD, miRNA expression is amenable to maximal ergospirometry and that the extent of changes differs between genders. Affected by exercise and gender were miRNAs that are associated, among others, with pathways for glucose metabolism, oxidative stress, and angiogenesis. Future studies should assess whether disease-specific miRNA expression in response to maximal exercise might serve as a marker for patient outcome.


Corresponding author: Prof. Josef Niebauer, MD, PhD, MBA, University Institute of Sports Medicine, Prevention and Rehabilitation and Research Institute of Molecular Sports Medicine and Rehabilitation, Paracelsus Medical University, Lindhofstr. 20, 5020 Salzburg, Austria, Phone: +43(0)5 7255 23200, Fax: +43(0)5 7255 23299, E-mail:

Acknowledgments

We thank all the participants for their time and effort they dedicated to this study.

  1. Research funding: None declared.

  2. Author contributions: BM, EEM, MS, JN contributed to the conception or design of the work. BM, EEM, CS, SD contributed to the acquisition, analysis, or interpretation of data for the work. BM drafted the manuscript. EEM, MS, CS, SD, JN critically revised the manuscript. All gave final approval and agree to be accountable for all aspects of work ensuring integrity and accuracy. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: Ethics Committee of the State of Salzburg no. 415-E/1734/10-2015, ClinicalTrials.gov: NCT02303379.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2021-0164).


Received: 2020-12-11
Accepted: 2021-05-04
Published Online: 2021-05-12
Published in Print: 2021-09-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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