Abstract
Objectives
Patient-based real-time quality control (PBRTQC) has gained increasing attention in the field of laboratory quality management in recent years. However, PBRTQC has not been reported for use in molecular diagnostics. This study introduces PBRTQC to quantitative hepatitis B virus (HBV) DNA test using moving rate (MR) of positive and negative patient results.
Methods
In contrast to the MR protocols described in other literature, MR protocol for HBV-DNA test has an additional logarithmic transformation and binary conversion steps before using a common statistical process control algorithm, such as the MR. We used all patient test results of HBV-DNA assay from August 2018 to August 2021 at the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, for parameters setting, optimization, and performance validation. The false rejection rate, error detection curves and validation charts were used to assess the MR protocols.
Results
The false rejection rates of two MR protocols were both <0.7%. The optimal block sizes for positive and negative errors in each cut-off value were not the same, so we first proposed a combined protocol that used different block size to detect negative and positive errors. It turned out that the combined protocols outperformed the simple protocols for each cut-off value, especially detecting positive errors.
Conclusions
The performances of MR protocols using positive or negative patient results to detect constant errors of HBV-DNA test could meet laboratory requirements. Therefore, we have provided an effective alternative tool for internal quality control in the field of molecular diagnostics.
Funding source: Guangzhou Basic and Applied Basic Research project
Award Identifier / Grant number: 202102020101
Funding source: Scientific research project of Traditional Chinese Medicine Bureau of Guangdong Province
Award Identifier / Grant number: 20202067
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Research funding: This work was supported by Scientific research project of Traditional Chinese Medicine Bureau of Guangdong Province (20202067) and Guangzhou Basic and Applied Basic Research project (202102020101).
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Author contributions: TL designed the study and wrote the manuscript; JL analyzed data. SC, YW and HW collected data; CZ reviewed the manuscript. PK proposed the concept of MR of positive patient results as a QC tool. XH supervised the study and reviewed the manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and have approved the submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: Not applicable.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2022-0338).
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