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Licensed Unlicensed Requires Authentication Published by De Gruyter December 30, 2022

Effects of lipemia on capillary serum protein electrophoresis in native ultra-lipemic material and intravenous lipid emulsion added sera

  • Mehmet Şeneş EMAIL logo , Medine Alpdemir , Gül Kırtıl , Elmas Öğüş , Emel Çolak Samsum and Mehmet Fatih Alpdemir

Abstract

Objectives

This study aims to investigate the effect of natural ultralipemic material (NULM) and intravenous lipid emulsion (IVLE) on capillary serum protein electrophoresis (SPEP).

Methods

NULM material was prepared from leftover patients’ lipemic serum sample (triglyceride concentration >2,000 mg/dL) pool by a refrigerated high-speed centrifuge, and IVLE Omegaven lipid emulsion (30%) was used. Serum pools for interference study were prepared from patient samples for which serum protein electrophoresis was studied as Normal SPEP and M Peak SPEP. For both types of lipemia (DULM and IVLE), five pools with triglyceride concentrations of ∼4.52 mmol/L, ∼7.91 mmol/L, ∼14.69 mmol/L, ∼21.47 mmol/L, and ∼28.25 mmol/L were prepared. SPEP was studied in each pool with Sebia Capillarys Minicap. A repeated measure ANOVA test was used to determine the difference between the pools, and interferograms were used to evaluate the interference effect.

Results

Interference was not detected in IVLE added Normal SPEP and M Peak SPEP pools, either % or concentrations of fractions. In NULM-added Normal SPEP and M Peak SPEP pools, significant positive interference in albumin % (p=0.002 and p<0.001 respectively) and significant negative interference in gamma% (p<0.001 and p=0.005 respectively) and M protein peak (p=0.002) fractions were detected. However, significant positive interference was seen only for albumin concentration fractions (p<0.001 for both pools).

Conclusions

It is vital to use NULM instead of IVLE solutions in lipemia interference studies for all laboratory tests, including CZE SPEP. The fractions concentration values calculated with the total protein concentration should be used for evaluating SPEP results.


Corresponding author: Mehmet Şeneş, Medical Biochemistry, University of Health Sciences Ankara Training and Research Hospital, Cebeci, 06340, Ankara, Türkiye, E-mail:

  1. Research funding: None declared.

  2. Author contributions: MS: wrote the manuscript; MS, EÇS: designed the study; EÖ, MA, GK, MFA: performed the sample collection and analysis; MS, EÇS: performed the statistical analysis; EÖ, MA, EÇS: critically revised the manuscript. All authors have accepted responsibility for the entire content of the submitted manuscript and approved submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The study protocol was prepared according to the Helsinki Declaration and approved by the University of Health Sciences Ankara Training and Research Hospital Clinical Research Ethics Committee (Decision No: 878/2022).

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/cclm-2022-0955).


Received: 2022-09-24
Accepted: 2022-12-15
Published Online: 2022-12-30
Published in Print: 2023-05-25

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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