Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter August 1, 2023

Epigenetic signatures of cfDNA enable a topological assignment of tissue damage

  • Catharina Gerhards EMAIL logo , Osman Evliyaoglu , Volker Ast and Michael Neumaier

Corresponding author: Dr. med. Catharina Gerhards, Institute for Clinical Chemistry, University Medical Center Mannheim, Heidelberg University, Medical Faculty Mannheim of the University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany, Phone: +0049 621/383 8414, Fax: 0049621/383 3819, E-mail:

Funding source: Ministry of Science, Research and Arts, Baden-Württemberg, Germany

Award Identifier / Grant number: Measures to combat the coronavirus SARS CoV-2 pandemic in the field of medical research, special funding programme COVID-19 (Chapt. 1499 TG93, MA10)

Acknowledgments

We thank Ameli Götz for aliquoting the samples and the isolation of cfDNA, Sonika Rao for conducting the routine laboratory data query and Caterina Hassler for recording of clinical symptoms. Moreover, we thank Annegret Reinschreid and Nadine Fricker (Institute of Human Genetics, Division of GenomicsLife & Brain, Research Centre, University Hospital of Bonn) for performing the epigenetic analyses. 

  1. Research ethics: The local Institutional Review Board deemed the study exempt from review. Institutional Review Board (2020-541N) approved the study protocol and study was conducted in accordance with the Declaration of Helsinki.

  2. Informed consent: Informed consent was obtained from all individuals included in this study.

  3. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  4. Competing interests: The authors state no Competing of interest.

  5. Research funding: Ministry of Science, Research and Arts, Baden-Württemberg, Germany: “Measures to combat the coronavirus SARS CoV-2 pandemic in the field of medical research”, special funding programme COVID-19 (Chap. 1499 TG 93), (MA10).

  6. Data availability: The data presented in this work are available on request from the corresponding author.

References

1. Alberry, MS, Aziz, E, Ahmed, SR, Abdel-Fattah, S. Non invasive prenatal testing (NIPT) for common aneuploidies and beyond. Eur J Obstet Gynecol Reprod Biol 2021;258:424–9. https://doi.org/10.1016/j.ejogrb.2021.01.008.Search in Google Scholar PubMed

2. Lo, YMD, Han, DSC, Jiang, P, Chiu, RWK. Epigenetics, fragmentomics, and topology of cell-free DNA in liquid biopsies. Science 2021;372:eaaw3616. https://doi.org/10.1126/science.aaw3616.Search in Google Scholar PubMed

3. Dedeurwaerder, S, Defrance, M, Calonne, E, Denis, H, Sotiriou, C, Fuks, F. Evaluation of the Infinium methylation 450K technology. Epigenomics 2011;3:771–84. https://doi.org/10.2217/epi.11.105.Search in Google Scholar PubMed

4. Moss, J, Magenheim, J, Neiman, D, Zemmour, H, Loyfer, N, Korach, A, et al.. Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease. Nat Commun 2018;9:5068. https://doi.org/10.1038/s41467-018-07466-6.Search in Google Scholar PubMed PubMed Central

5. Verity, R, Okell, LC, Dorigatti, I, Winskill, P, Whittaker, C, Imai, N, et al.. Estimates of the severity of coronavirus disease 2019: a model-based analysis. Lancet Infect Dis 2020;20:669–77. https://doi.org/10.1016/s1473-3099(20)30243-7.Search in Google Scholar PubMed PubMed Central

6. Dudley, JP, Lee, NT. Disparities in age-specific morbidity and mortality from SARS-CoV-2 in China and the Republic of Korea. Clin Infect Dis 2020;71:863–5. https://doi.org/10.1093/cid/ciaa354.Search in Google Scholar PubMed PubMed Central

7. Leitzmann, MF, Rohrmann, S. Risk factors for the onset of prostatic cancer:age, location, and behavioral correlates. Clin Epidemiol 2012;4:1–11. https://doi.org/10.2147/clep.s16747.Search in Google Scholar

Received: 2023-06-26
Accepted: 2023-07-16
Published Online: 2023-08-01
Published in Print: 2024-01-26

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 9.5.2024 from https://www.degruyter.com/document/doi/10.1515/cclm-2023-0744/html
Scroll to top button